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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has become observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial position in marketing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two extra splice variants were being documented but comprehensive nucleotide sequence data is not nevertheless offered.

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Blocking peptides are peptides that bind particularly to the focus on antibody and block antibody binding. These peptide ordinarily contains the epitope acknowledged because of the antibody.

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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...

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This guideline outlines further more attainable optimization of mobile and immunoassay parameters to make sure the very best results are...

Antibodies certain to the blocking peptide not bind to your epitope on the focus on protein. By comparing the staining within the blocked antibody compared to the antibody by itself, one can see which staining is specific.

Concentrate on Details Cyclin E belongs into the extremely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit check here distinct expression and degradation patterns which add towards the temporal coordination of every mitotic party. Cyclin E types a posh with and features as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates with the G1-S phase boundary which is degraded as cells progress by means of S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...

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